Production of L-tyrosinase from Novel Variants of Streptomyces cellulosae

Abstract: Background: Recently, a lot of interest has been focused on the discovery high L-tyrosinaseyielding microorganisms that catalyze production enantiomerically pure L-DOPA, which recognized as conventional treatment for Parkinson's disease. Objectives: The current work primarily isolating novel, promising tyrosinase-producing isolates and performing taxonomical characterization them. Materials Methods: Initially six various actinomycetes were used in present study. Primary screening secondary selected performed. By evaluating morphological, cultural, physiological, biochemical molecular characterization, identified. Using 16S rRNA gene sequencing, isolates' Phylogenetic investigations conducted. Results: Streptomyces cellulosae variants, PD18 PD26 identified to be potential actinomycete their respective maximal tyrosinase yields reported 21.20 IU/mL 24.19 IU/mL. Conclusion: According observations experiment, SS (Soluble starch) medium with 1mg/mL copper sulphate was most suitable from our PD26. maximum yield noticed after 168 hr incubation. This emphasizes significance finding novel variants cellulosae, first produce well sources potentially bioactive substances like antimicrobial metabolites, biosurfactants enzymes amylase, lipase, cellulase, uricase, urease, β- galactosidase L- asparaginase. Keywords: Tyrosinase, tyrosine, medium, sulphate, cellulosae.